心肌炎麻疹病毒IgG免疫診斷試劑盒

英文名稱：American FUCUS measles virus diagnostic kit

廣州健侖生物科技有限公司

（廣州健侖生物科技有限公司是集研制開發(fā)、銷售、服務(wù)于一體的優(yōu)良企業(yè),公司產(chǎn)品涉及臨床快速診斷試劑、食品安全檢測(cè)試劑，違禁品快速檢測(cè)，動(dòng)物疾病防疫檢測(cè)試劑，免疫診斷試劑、臨床血液學(xué)和體液學(xué)檢驗(yàn)試劑、微生物檢驗(yàn)試劑、分子生物學(xué)檢驗(yàn)試劑、臨床生化試劑、有機(jī)試劑等眾多領(lǐng)域，同時(shí)核心代理Panbio、FOCUS、Qiagen、IBL、CORTEZ、Fuller、Inbios、BinaxNOW、LumuQuick、日本富士、日本生研等多家有名診斷產(chǎn)品集團(tuán)公司產(chǎn)品，致力于為商檢單位、疾病預(yù)防控制中心、海關(guān)出入境檢疫局、衛(wèi)生防疫單位,緝毒系統(tǒng)，戒毒中心，檢驗(yàn)檢疫單位、生化企業(yè)、科研院所、醫(yī)療機(jī)構(gòu)等機(jī)構(gòu)與行業(yè)提供*、高品質(zhì)的產(chǎn)品服務(wù)。此外，本公司還開展食品、衛(wèi)生、環(huán)境、藥品等多方面的第三方檢測(cè)服務(wù)。）

主要用途：用于定量測(cè)定人血清、腦脊液或血漿中的麻疹抗體。

產(chǎn)品規(guī)格：96T/盒

存儲(chǔ)條件：4-8℃

保質(zhì)期：18個(gè)月

【麻疹的介紹】

麻疹病毒的*自然儲(chǔ)存宿主是人。急性期患者是傳染源，患者在出疹前6天至出疹后3天有傳染性。通過飛沫傳播、也可經(jīng)用具、玩具或密切接觸傳播。麻疹傳染性*，易感者接觸后幾乎全部發(fā)病。發(fā)病的潛伏期為9～12天。由于CD46是麻疹病毒受體，因此具有CD46的大多組織細(xì)胞均可為麻疹病毒感染的靶細(xì)胞。經(jīng)呼吸道進(jìn)入的病毒首先與呼吸道上皮細(xì)胞受體結(jié)合并在其中增殖，繼之侵入淋巴結(jié)增殖，然后入血（在白細(xì)胞內(nèi)增殖良好），形成*次病毒血癥。病毒到達(dá)全身淋巴組織大量增殖再次入血，形成第二次病毒血癥。此時(shí)開始發(fā)熱，繼之由于病毒在結(jié)膜、鼻咽粘膜和呼吸道粘膜等處增殖而出現(xiàn)上呼吸道卡他癥狀。病毒也在真皮層內(nèi)增殖，口腔兩頰內(nèi)側(cè)粘膜出現(xiàn)中心灰白、周圍紅色的Koplik斑，3天后出現(xiàn)特征性皮疹，皮疹形成的原因主要是局部產(chǎn)生超敏反應(yīng)。一般患兒皮疹出齊24小時(shí)后，體溫開始下降，呼吸道癥狀一周左右消退，皮疹變暗，有色素沉著。有些年幼體弱的患兒，易并發(fā)細(xì)菌性感染，如繼發(fā)性支氣管炎、中耳炎，尤其易患細(xì)菌性肺炎，這是麻疹患兒死亡的主要原因。大約有0.1%的患者發(fā)生腦脊髓炎，它是一種遲發(fā)型超敏反應(yīng)性疾病，常于病愈1周后發(fā)生，呈典型的脫髓鞘病理學(xué)改變及明顯的淋巴細(xì)胞浸潤(rùn)，常留有*性后遺癥，病死率為15%。免疫缺陷兒童感染麻疹病毒，常無(wú)皮疹，但可發(fā)生嚴(yán)重致死性麻疹巨細(xì)胞肺炎。百萬(wàn)分之一麻疹患者在其恢復(fù)后若干年，多在學(xué)齡期前出現(xiàn)亞急性硬化性全腦炎（subacute sclerosing panencephalitis，SSPE）。SSPE屬急性感染的遲發(fā)并發(fā)癥，表現(xiàn)為漸進(jìn)性大腦衰退，1～2年內(nèi)死亡。經(jīng)研究發(fā)現(xiàn)，患者血清及腦脊液中雖有高效價(jià)的IgG或IgM抗麻疹病毒抗體，但是用這些抗體很難分離出麻疹病毒。現(xiàn)認(rèn)為腦組織中的病毒為麻疹缺陷病毒，由于在腦細(xì)胞內(nèi)病毒M基因變異而缺乏合成麻疹病毒M蛋白的能力，從而影響病毒的裝配、出芽及釋放。因此，將SSPE尸檢腦組織細(xì)胞與對(duì)麻疹病毒敏感細(xì)胞（如HeLa、Vero等）共同培養(yǎng)，可分離出麻疹病毒。

【注意事項(xiàng)】　　

Only the complete use of reagents to ensure that the test results, because all reagents are related, can not be mixed with other manufacturers of products. Especially standard serum, control serum and enzyme markers, must be equipped with the kit, do not use other batch products. Dilution buffer, wash solution, stop solution, and substrate solution are used for all Serun ELISA classic kits.
 
All reagents in the ELISA classic kit must be properly stored. It should be stored at 2-8 ° C in the unopened condition and used for a period of validity (see label instructions). Detailed stability and storage information will be described in detail in "8. Storage and Stability".
 
Each reagent is calibrated to ensure optimal test results. The lack of regulation or dilution of these reagents can lead to a decrease in the sensitivity of the assay.
 
Avoid exposing reagents to bright light during storage and incubation. All reagent bottle caps should be tightly closed to prevent evaporation and contamination and reagents should be free from microbial contamination as proteolytic enzyme interference will result in erroneous results.
 
Please cut the sealed bag from the mark. If the aluminum bag is damaged or the aluminum bag with the desiccant is opened but not closed by the clip, do not continue to use the microwell.
 
Allow all reagents to room temperature before starting the test.
 
When taking reagents from the reagent tube, care should be taken to use aseptic technique to prevent contamination. To avoid false-positive results, aspiration of the enzyme conjugate, the nozzle should be kept out of contact with the eyelet or sprayed on it, taking care not to mistake the cap or cap.
 
The repeatability of the test results depends on the well-mixed reagents. Shake the vial containing control serum and all diluted solution (eg using a mixer) before use.
 
Carefully pipet the reagents and strictly observe the given incubation time and temperature. Note that when the specimen / control serum, enzyme conjugate, or substrate is aspirated, the time interval between the first well and the last well sample will result in a different "pre-incubation" time, Affect the accuracy and repeatability of the measured values.
 
If the test does not strictly abide by the validity of the control quality control certification documents specific guidelines, the test results invalid.
 
Inadequate washing will affect the test results:
 
Care should be taken to wash. The washing procedure should be carried out in accordance with the instruction manual for the relevant scrubber (flat bottom hole, hole diameter 7 mm, depth 10.9 mm). It is important that all wells be filled with the same volume of wash buffer. At the end of the wash, the microplate should be back-loaded onto a paper towel and tapped gently to ensure that no wash buffer is present in all wells. Avoid bubbles! If using an automatic plate washer, pay attention to correct operation.

【檢測(cè)原理】

ELISA（酶聯(lián)免疫吸附測(cè)定）是涉及的免疫學(xué)過程在抗體檢測(cè)的感染領(lǐng)域尤其得到證實(shí)。該基于抗體和抗原的特異性相互作用的檢測(cè)反應(yīng)。至為此目的，使用賽潤(rùn)ELISA classic的微量滴定板的測(cè)試條傳染性病原體特異性抗原在患者樣品中的結(jié)合包被的抗體存在。 其他用堿性磷酸酶標(biāo)記二抗檢測(cè)由此形成的免疫復(fù)合物。 該酶催化a反應(yīng)過程中，無(wú)色底物對(duì)硝基苯磷酸酯在有色產(chǎn)物中對(duì)硝基苯酚轉(zhuǎn)化。 反應(yīng)產(chǎn)物的信號(hào)強(qiáng)度正比于樣品中的抗體濃度用光度法檢測(cè)。

心肌炎麻疹病毒IgG免疫診斷試劑盒

【試劑盒的組成】

我司同時(shí)還提供、美國(guó)FOCUS、西班牙DIA、美國(guó)trinity等試劑盒：

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The only natural storage host for measles is human. Acute phase is the source of infection, the patient 6 days before the rash to 3 days after the rash contagious. Spread through the droplets can also be used toys, toys or close contact with the spread. Highly contagious measles, susceptible contacts almost all of the disease. The incubation period of 9 to 12 days. Because CD46 is a measles virus receptor, most of the tissue cells that have CD46 can be target cells for measles virus infection. The virus that enters the respiratory tract first binds to and multiplies in the respiratory epithelial cell receptors, subsequently invades the lymph nodes and proliferates, then enters the bloodstream (proliferating well in leukocytes), forming the first viremia. The virus reached the body mass proliferation of lymphoid tissue into the blood again, the formation of the second viremia. At this point began fever, followed by the virus in the conjunctiva, nasopharyngeal mucosa and respiratory tract proliferation and other symptoms appear on the upper respiratory tract catarrhal. The virus also proliferated in the dermis. The inner mucosa of the buccal cavity appeared grayish center and surrounded by red Koplik spots. The characteristic rashes appeared after 3 days. The main cause of the rash was local hypersensitivity. General children rash out Qi 24 hours after the temperature began to decline, respiratory symptoms subsided about a week, the rash darker, pigmentation. Some young infirm children, complicated by bacterial infections, such as secondary bronchitis, otitis media, especially susceptible to bacterial pneumonia, which is the main cause of death in children with measles. About 0.1% of patients with encephalomyelitis, it is a delayed-type hypersensitivity disease, often occurs after a week of recovery, showing typical demyelinating pathology and lymphocyte infiltration, often A permanent sequelae, the case fatality rate was 15%. Children with immunodeficiency infection with measles virus, often without rash, but can occur severe lethal measles giant cell pneumonia. Measles patients one millionth of measles have subacute sclerosing panencephalitis (SSPE) more than preschool age years after their recovery. SSPE is a late-onset complication of acute infection, manifested as progressive brain failure, death within 1 to 2 years. The study found that patients with serum and cerebrospinal fluid despite high titers of IgG or IgM anti-measles virus antibodies, but with these antibodies is difficult to isolate the measles virus. It is thought that the virus in the brain tissue is a measles-deficient virus, which lacks the ability to synthesize the M protein of measles virus due to the variation of the viral M gene in the brain cells, thereby affecting the assembly, budding and release of the virus. Therefore, the SSPE autopsy brain tissue cells and measles virus-sensitive cells (such as HeLa, Vero, etc.) co-culture, measles virus can be isolated.