流感病毒A+B診斷試劑盒（快檢方法）

廣州健侖生物科技有限公司

廣州健侖長期供應各種流感檢測試劑，包括進口和國產(chǎn)的品牌，主要包括日本富士瑞必歐、日本生研、美國BD、美國NovaBios、美國binaxNOW、凱必利、廣州創(chuàng)侖等主流品牌。

流感病毒A+B診斷試劑盒（快檢方法）

我司還提供其它進口或國產(chǎn)試劑盒：登革熱、瘧疾、流感、A鏈球菌、合胞病毒、腮病毒、乙腦、寨卡、黃熱病、基孔肯雅熱、克錐蟲病、違禁品濫用、肺炎球菌、軍團菌、化妝品檢測、食品安全檢測等試劑盒以及日本生研細菌分型診斷血清、德國SiFin診斷血清、丹麥SSI診斷血清等產(chǎn)品。

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【公司名稱】 廣州健侖生物科技有限公司
【市場部】    楊永漢

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【騰訊  】 2042552662
【公司地址】 廣州清華科技園創(chuàng)新基地番禺石樓鎮(zhèn)創(chuàng)啟路63號二期2幢101-103室

注意事項
抗原修復時必須保證切片始終能浸泡在液體內(nèi)，一定要等工作液冷卻后才能將切片取出
本試劑盒應用雙抗體夾心法測定標本中犬組織因子（TF）水平。用純化的犬組織因子（TF）抗體包被微孔板，制成固相抗體，往包被單抗的微孔中加入組織因子（TF），再與HRP標記的組織因子（TF）抗體結(jié)合，形成抗體-抗原-酶標抗體復合物，經(jīng)過*洗滌后加底物TMB顯色。TMB在HRP酶的催化下轉(zhuǎn)化成藍色，并在酸的作用下轉(zhuǎn)化成zui終的黃色。顏色的深淺和樣品中的組織因子（TF）呈正相關(guān)。用酶標儀在450nm波長下測定吸光度（OD值），通過標準曲線計算樣品中犬組織因子（TF）的含量。
樣本處理及要求：
1. 血清：室溫血液自然凝固10-20分鐘，離心20分鐘左右（2000-3000轉(zhuǎn)/分）。仔細收集上清，保存過程中如出現(xiàn)沉淀，應再次離心。
2. 血漿：應根據(jù)標本的要求選擇EDTA或檸檬酸鈉作為抗凝劑，混合10-20分鐘后，離心20分鐘左右（2000-3000轉(zhuǎn)/分）。仔細收集上清，保存過程中如有沉淀形成，應該再次離心。
3. 尿液：用無菌管收集，離心20分鐘左右（2000-3000轉(zhuǎn)/分）。仔細收集上清，保存過程中如有沉淀形成，應再次離心。胸腹水、腦脊液參照實行。

Precautions
Antigen repair must ensure that the slice can always be immersed in the liquid, must wait for the cooling of the working fluid before the slice can be removed
This kit uses double antibody sandwich method to determine the level of canine factor (TF) in the sample. Principle of the assay The kit assay Human TF level in the sample, use Purified Canine Tissue Factor (TF) antibody to coat microtiter plate wells, make solid-phase antibody, and then add tissue factor (TF) to the wells of the coating. Then bind to HRP-labeled tissue factor The formation of antibody - antigen - enzyme antibody complex, after thorough washing with substrate TMB color. TMB is converted to blue by the HRP enzyme catalysis and converted to the final yellow color by the action of an acid. The color depth is positively correlated with the tissue factor (TF) in the sample. The absorbance (OD value) was measured with a microplate reader at a wavelength of 450 nm, and the content of canine factor (TF) in the sample was calculated by a standard curve.
Sample Processing and Requirements:
1. Serum: blood coagulation at room temperature 10-20 minutes, centrifuged for about 20 minutes (2000-3000 r / min). Carefully collect the supernatant, if precipitation occurs during storage, centrifuge again.
2. Plasma: EDTA or sodium citrate should be selected as an anticoagulant according to the specimen's requirements. After mixing for 10-20 minutes, centrifuge for about 20 minutes (2000-3000 rpm). Carefully collect the supernatant, if precipitation is formed during the storage, it should be centrifuged again.
3. Urine: Collect with sterile tube and centrifuge for about 20 minutes (2000-3000 rpm). Carefully collect the supernatant, if precipitation is formed during the storage, centrifuge again. Pleural effusion, cerebrospinal fluid reference implementation.