SEKISUI流感病毒抗原快檢試劑盒

廣州健侖生物科技有限公司

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SEKISUI流感病毒抗原快檢試劑盒

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【公司名稱】 廣州健侖生物科技有限公司
【市場部】    楊永漢

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【騰訊  】 2042552662
【公司地址】 廣州清華科技園創新基地番禺石樓鎮創啟路63號二期2幢101-103室

石蠟切片免疫組化染色步驟
1、載玻片的處理：
    抗原修復過程中，由于高溫、高壓、輻射等諸多因素的影響，極易造成脫片。為保證試驗的正常進行，可選用我公司提供的ZLI-9001 APES、ZLI-9003 HistogripTM或ZLI-9005 Poly-L-Lysine 等幾種試劑，對已清洗的載玻片進行處理。具體方法如下：
1.1 APES：現用現配。將洗凈的玻片放入以1:50 比例丙酮稀釋的APES 中，停留20~30 秒鐘，取出稍停片刻，再入純丙酮溶液或蒸餾水中涮去未結合的APES，置通風櫥中晾干即可。用此載玻片撈片時應注意組織要一步到位，并盡量減少氣泡的存在，以免影響染色結果。
1.2 HistogripTM：將洗凈的玻片放入以1:50比例丙酮稀釋的Histogrip液中，停留1~2 分鐘，然后用雙蒸水快速清洗三次，室溫干燥或60oC 烤箱烘烤一小時，裝盒備用。
1.3 Poly-L-Lysine：將洗凈、干燥的載玻片放入以1:10 比例去離子水稀釋的多聚賴氨酸溶液中，浸泡5 分鐘，60oC 烤箱烘烤一小時或室溫過夜干燥。裝盒備用。試驗中使用的器具均為非玻璃制品。
2、常用酶消化：
2.1 胰蛋白酶：一般使用濃度為0.05%~0.1%，消化時間為37℃、10~40 分鐘，主要用于細胞內抗原的顯示。
2.2 胃蛋白酶：一般使用濃度為0.4%，消化時間為37℃、30~180 分鐘，主要用于細胞間質抗原的顯示，如：Laminin（層粘蛋白），Collagen IV（IV型膠原）等。
2.3 皂素（Saponin）：一般使用濃度為2~10?g/ml 的saponin 溶液，消化時間為室溫孵育30 分鐘。

Paraffin sections immunohistochemical staining steps
1, slide processing:
    Antigen recovery process, due to high temperature, high pressure, radiation and many other factors, can easily lead to delamination. To ensure the normal operation of the test, the slides cleaned can be processed by using several reagents such as ZLI-9001 APES, ZLI-9003 HistogripTM or ZLI-9005 Poly-L-Lysine provided by our company. Specific methods are as follows:
1.1 APES: Available now with. Place the washed glass slide in APES diluted with acetone 1:50 and leave it for 20-30 seconds. Take out the pause and re-enter the pure acetone solution or distilled water to rinse the unbound APES and set in a fume hood Can be dried. Use this slides slide should pay attention to the organization to be one step, and minimize the presence of bubbles, so as not to affect the staining results.
1.2 HistogripTM: Wash the slides in a 1:50 dilution of Histogrip in acetone for 1 to 2 minutes, rinse quickly with double distilled water for three times, dry at room temperature or bake in a 60oC oven for 1 hour spare.
1.3 Poly-L-Lysine: Place the washed and dried slide in a polylysine solution diluted 1:10 in deionized water, soak for 5 minutes, bake in a 60oC oven for one hour or overnight at room temperature . Cartoning spare. The test equipment used are non-glass products.
2, commonly used enzyme digestion:
2.1 trypsin: general use concentration of 0.05% to 0.1%, the digestion time of 37 ℃, 10 to 40 minutes, mainly for the display of intracellular antigens.
2.2 pepsin: the general use of 0.4%, the digestion time of 37 ℃, 30 to 180 minutes, mainly for the display of interstitial antigens, such as: Laminin (laminin), Collagen IV (type IV collagen) and so on.
2.3 Saponin: Saponin is generally used at a concentration of 2 to 10 μg / ml and the digestion time is 30 minutes at room temperature.